Its development starts with the formation of the posterior primary primordium (PrimI), which arises from the disintegration of the posterior primary sensory placode ( Gompel et al., 2001 Nikaido et al., 2017). The zebrafish posterior lateral line system (pLLS) is a unique model for studying collective cell migration as well as axonal outgrowth and pathfinding during development of a sensory organ ( Metcalfe et al., 1985 Kimmel et al., 1995 Gilmour et al., 2002 Dambly-Chaudière et al., 2007 Aman and Piotrowski, 2008 Lush and Piotrowski, 2014 Romero-Carvajal et al., 2015). Finally, we show that the knockdown of ncam1b destabilizes proneuromasts, suggesting a further function of Ncam1b in strengthening the cohesion of proneuromast cells. In addition, we demonstrate that Ncam1b is required for the expression of chemokine receptor Cxcr7b, which is crucial for directed primordial migration. We conclude that Ncam1b is involved in activating proliferation by triggering the expression of erm. Ncam1b directly interacts with Fgf receptor Fgfr1a, and a knockdown of fgfr1a causes similar phenotypic changes as observed in ncam1b-morphants. We find that morpholino-knockdowns of ncam1b (i) reduce cell proliferation within the primordium, (ii) reduce the expression of Fgf target gene erm, (iii) severely affect proneuromast formation, and (iv) affect primordium migration. We here show, that the Neural Cell Adhesion Molecule Ncam1b is an integral part of the pathways initiating and regulating the development of the pLLS in zebrafish. They develop from proneuromasts, which are deposited from a migratory primordium on its way to the tip of the tail. The posterior lateral line system (pLLS) of aquatic animals comprises small clustered mechanosensory organs along the side of the animal. Zoological Institute, Cell- and Neurobiology, Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany.Berghaus, Martin Bastmeyer and Joachim Bentrop * For geneticists, biologists, and especially students, however, it's a versatile, compatible lab-grade research tool.Ramona Dries, Annemarie Lange, Sebastian Heiny, Katja I. It's easy to use, but it requires specific knowledge to make proper use of it.
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